A comprehensive investigation on cells and colony motion offers new insight into the proliferative and epithelial regenerative capacities of human primary oral keratinocyte cultures with implications for quality control of engineered cells used in regenerative medicine. Dr. Kenji Izumi and his colleagues, Dr. Emi Hoshikawa and Dr. Taisuke Sato, modified the optical flow (OF) protocol originally presented in their 2019 paper to add the capacity to determine the threshold of the cells/colony motion speed required to differentiate substandard oral keratinocyte populations before manufacturing a tissue-engineered oral mucosa tissue construct.